Karyology and agar analysis of the agarophyte <i>Gelidiella acerosa</i> (Forsskål) Feldmannet Hamel from the Philippines

Abstract

Microspectrophotometry with the DNA-localizing fluorochrome DAPI demonstrated ploidy level differences in tetrasporophytic and presumptive gametophytic phases of Gelidiella acerosa from the Philippines. Comparison of mean nuclear DNA (I f ) values to chicken erythrocytes (RBC) resulted in an estimate of 0.32 pg/2 C genome. Karyological studies with aceto-orcein revealed a chromosome complement of six bivalents during diakinesis of tetrasporocytes. The agar yield ranges from 13–24% dry weight, depending on the method of extraction. Agar extraction in 1 N NaOH resulted in an increased gel strength of 189 g cm−2 at 1.5% concentration. Infrared spectroscopy indicated a relatively high sulfate content in native agar. The low (61 °) melting temperature is indicative of high sulfation and small molecular size.