National Committee on Marine Sciences (NCMS)
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- Chemical characteristics and gelling properties of agar from two Philippine Gracilaria spp. (Gracilariales, Rhodophyta)Montaño, Nemesio E.; Villanueva, Ronald D.; Romero, Jumelita B. (Springer, 1999)The chemical structure of agars extracted from Philippine Gracilaria arcuata and G. tenuistipitata were determined by NMR and infrared spectroscopy. Agar with alternating 3-linked 6-O-methyl-β-D-galactopyranosyl and 4-linked 3,6-anhydro-2- O-methyl-α-L-galactopyranosyl units was isolated from G. arcuata, while the agar from G. tenuistipitata possesses the regular agarobiose repeating unit with partial methylation at the 6-position of the D-galactosyl residues. Both agars exhibit sulphate substitution at varying positions in the polymer. Chemical analyses reveal higher 3,6-anhydrogalactose and lower sulphate contents in alkali-modified than in native agar from both samples. Also, alkali modification enhanced agar gel strength and syneresis. Native G. arcuata agar produces a viscous solution (2000 cP at 75 °C) with a high gelling point (>60 °C) that forms a soft gel even after alkali modification (gel strength: <300 g cm−2). On the other hand, the agar from G. tenuistipitata exhibits gel qualities typical of most Gracilaria agars.
- Karyology and agar analysis of the agarophyte Gelidiella acerosa (Forsskål) Feldmannet Hamel from the PhilippinesKapraun, Donald F.; Ganzon-Fortes, Edna; Bird, Kimon T.; Trono, Gavino; Breden, Chris (Springer, 1994-12)Microspectrophotometry with the DNA-localizing fluorochrome DAPI demonstrated ploidy level differences in tetrasporophytic and presumptive gametophytic phases of Gelidiella acerosa from the Philippines. Comparison of mean nuclear DNA (I f ) values to chicken erythrocytes (RBC) resulted in an estimate of 0.32 pg/2 C genome. Karyological studies with aceto-orcein revealed a chromosome complement of six bivalents during diakinesis of tetrasporocytes. The agar yield ranges from 13–24% dry weight, depending on the method of extraction. Agar extraction in 1 N NaOH resulted in an increased gel strength of 189 g cm−2 at 1.5% concentration. Infrared spectroscopy indicated a relatively high sulfate content in native agar. The low (61 °) melting temperature is indicative of high sulfation and small molecular size.
- Quantification and characterization of nuclear genomes in commercial red seaweeds (Gracilariales) from the PhilippinesKapraun, Donald F.; Lopez-Bautista, Juan; Trono, Gavino; Bird, Kimon T. (Springer, 1996-03)Eight species of Gracilariaceae from the Philippines, representing the genera Gracilaria, Gracilariopsis and Hydropuntia, were investigated to quantify and characterize their nuclear genomes. DNA reassociation kinetics were used to determine nuclear genome organization and complexity in six of these species. Results indicate the presence of three second order components corresponding to fast, intermediate and slow fractions. Repetitive sequences varied from 13–74% and unique DNA ranged from 26–84%. Microspectrophotometry with the DNA-localizing fluorochrome DAPI was used to quantify nuclear DNA contents. Comparisons of mean nuclear DNA (I f ) values to chicken erythrocytes (RBC) resulted in an estimate of 0.38–0.43 pg/2 C genomes for seven of the species investigated. Preliminary analyses of agar content and quality confirm the economic potential of Gracilaria firma, Gracilaria sp. 2 from Sorsogon and Gracilariopsis bailinae. Nuclear genome profiles developed from data for genome size, organization and complexity are compared with data for agar quantity and quality. Gel quality and quantity do not appear to be correlated with either large repetitive fraction DNA or a high degree of genome complexity.
- Fisheries Administrative Order No. 245-4: Series of 2018. Regulations and implementing guidelines on group tuna purse seine operations in high seas pocket number 1 as a special management area.(Department of Agriculture, 2018-07-25)This Administrative Order covers the 36 Philippine registered traditional group seine fishing vessels granted access to the HSP1-SMA, having gross tonnage of not more than 250 GT issued with International Fishing Permits, and listed in the WCPFC record of fishing vessels. It applies only to HSP-1 SMA, which is the area of the high seas bounded by the EEZs of the Federated States of Micronesia to the north and east, Republic of Palau to the west, Indonesia and Papua New Guinea to the south with exact coordinates as used by WCPFC Vessel Monitoring. Fishing access to operate in HSP1-SMA is granted on the basis of compliance to specific criteria. Compatible measure for the high seas and exclusive economic zones (EEZs) are implemented so that bigeye, yellowfin and skipjack tuna stocks are, at a minimum,maintained at levels capable of producing their maximum sustainable yield, as qualified by relevant environmental and economic factors including the special requirements of developing States in the Convention Area as expressed by Article 5 of the Convention. The vessels or the fishing company shall notify BFAR through electronic or any means the entry and the exit the HSP-1 SMA. Likewise, this information shall be transmitted to the adjacent coastal States / Territories and WCPFC. The report should be in the following format: "VID/entry or Exit: Date/Time; Lat/Long." The vessels operating in HSP-1 SMA should report sightings of any fishing vessel to the BFAR and WCPFC Secretariat Such information shall include vessel type, date, time, position, markings, heading and speed. The Bureau shall maintain an updated list of all fishing vessels operating in the I-ISP-i SMA based on the foregoing vessel's entry and exit reports submitted to WCPFC. The list will be submitted to WCPFC and will be made available to WCPFC members and other concerned stakeholders through dedicated website, www.bfar.gov.ph. Any violations of the provisions of this Fisheries Administrative Order shall be penalized with fines and penalties specified in the text.
- Fisheries Administrative Order No. 227: Series of 2008. Rules and regulations governing the export of fish and aquatic products to European Union member countries.(Department of Agriculture, 2008-11-20)This Order, consisting of 11 articles, establishes Rules and Regulations governing the Export of Fish and Live Aquatic Animals Products to European Member Countries. This Order lays down specific rules on the hygiene of processed and unprocessed FA products for business operators intending to export their products to the EU for human consumption. Unless specifically stated to the contrary, this Order does not apply to processed foods containing both products of plant origin and processed FA products. However, any processed FA products used to prepare such foods must be obtained and handled in accordance with the requirements of this Order. This Order must apply without prejudice to the general requirements of Republic Act No 8550, Philippine Fisheries Code of 1998, and the following FAOs as applied to the harvesting, production, preparation and processing of FA products for domestic or non-EU markets: The Fishery and Aquaculture Business Operator Responsibilities (FABO) must comply with the requirements of the relevant provisions of the PD 856 - Code of Sanitation of the Philippines - and such additional provisions of this Order that are required by EU legislation from time to time in force. FABO must also comply with the relevant provisions in Chapters I to V of the Implementing Code of Practice for this Order ("The Code of GMP for Business Operators Exporting FA Products from the Philippines to the EU, 2008") and take all steps necessary to meet the food safety objectives set by this Order. In addition, FABO must ensure that all processed FA products exported to the EU comply with all relevant EU food safety legislation, in particular that relating to: (i) specified maximum levels for certain contaminants such as histamine, heavy metals, dioxins and PCB-like materials, and poly-cyclic aromatic hydrocarbons, set out in Chapter III, Section II, Part VII of the Code of Practice referred to in paragraph 2; (ii) microbiological criteria; (iii) permitted veterinary drugs and maximum residues arising from their use; (iv) food contact materials; and (v) permitted food additives and their conditions of use.
- Fisheries Administrative Order No. 226: Series of 2008. Regulation on the mesh size of tuna purse seine nets and trading of small tuna.(Department of Agriculture, 2008-08-01)This Administrative Order, consisting of 6 Sections, establishes the Regulation on the Mesh Size of Tuna Purse Seine Nets and Trading of Small Tuna. The following management and conservation measures to prescribe the mesh size of tuna purse seine nets in catching tuna and regulation on the trading of small tuna are promulgated to prohibit to any person, association, cooperative, partnership or corporation to operate tuna purse seine nets with mesh size smaller than 3.5 inches (8.89 cm) at the bag or bunt portion in catching tuna. It shall be unlawful also to trade small tuna caught beyond the bycatch ceiling. Violation of Section 2 of this order shall subject the offender to a fine of from 2,000.00 to 20,000.00 Pesos or imprisonment from six months to two years or both such fine and imprisonment at the discretion of the court; the boat captain and the master fisherman may also be subject to the penalties provided; the owner/operator of the commercial fishing vessel who violates this provision shall be subjected to the same penalties; the Department is empowered to impose upon the offender an administrative fine and/or cancel the fishing licence.