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National Committee on Marine Sciences (NCMS)

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Author: search.filters.author.Bird, Kimon T.SDG: search.filters.sdg.SDG 14 - Life below water

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    Karyology and agar analysis of the agarophyte Gelidiella acerosa (Forsskål) Feldmannet Hamel from the Philippines
    Kapraun, Donald F.; Ganzon-Fortes, Edna; Bird, Kimon T.; Trono, Gavino; Breden, Chris (Springer, 1994-12)
    Microspectrophotometry with the DNA-localizing fluorochrome DAPI demonstrated ploidy level differences in tetrasporophytic and presumptive gametophytic phases of Gelidiella acerosa from the Philippines. Comparison of mean nuclear DNA (I f ) values to chicken erythrocytes (RBC) resulted in an estimate of 0.32 pg/2 C genome. Karyological studies with aceto-orcein revealed a chromosome complement of six bivalents during diakinesis of tetrasporocytes. The agar yield ranges from 13–24% dry weight, depending on the method of extraction. Agar extraction in 1 N NaOH resulted in an increased gel strength of 189 g cm−2 at 1.5% concentration. Infrared spectroscopy indicated a relatively high sulfate content in native agar. The low (61 °) melting temperature is indicative of high sulfation and small molecular size.
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    Quantification and characterization of nuclear genomes in commercial red seaweeds (Gracilariales) from the Philippines
    Kapraun, Donald F.; Lopez-Bautista, Juan; Trono, Gavino; Bird, Kimon T. (Springer, 1996-03)
    Eight species of Gracilariaceae from the Philippines, representing the genera Gracilaria, Gracilariopsis and Hydropuntia, were investigated to quantify and characterize their nuclear genomes. DNA reassociation kinetics were used to determine nuclear genome organization and complexity in six of these species. Results indicate the presence of three second order components corresponding to fast, intermediate and slow fractions. Repetitive sequences varied from 13–74% and unique DNA ranged from 26–84%. Microspectrophotometry with the DNA-localizing fluorochrome DAPI was used to quantify nuclear DNA contents. Comparisons of mean nuclear DNA (I f ) values to chicken erythrocytes (RBC) resulted in an estimate of 0.38–0.43 pg/2 C genomes for seven of the species investigated. Preliminary analyses of agar content and quality confirm the economic potential of Gracilaria firma, Gracilaria sp. 2 from Sorsogon and Gracilariopsis bailinae. Nuclear genome profiles developed from data for genome size, organization and complexity are compared with data for agar quantity and quality. Gel quality and quantity do not appear to be correlated with either large repetitive fraction DNA or a high degree of genome complexity.