Journal Articles - UP - MSI
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- Wound healing in cultured Eucheuma alvareziivar. tambalang DotyAzanza-Corrales, R.; Dawes, C. J. (Walter de Gruyter GmbH, 1989)Wound healing in segments of Eucheuma alvarezii var. tambalang grown in enriched media in the laboratory can be divided into four stages based on histological and cytological changes. During the first stage, approximately 2—4 days after wounding, proteinaceous and phenolic substances concentrate on pit plugs of cells adjacent to the wounded surface. In the second stage, about the sixth day, cellular extensions are produced from the pits of medullary and cortical cells of sub-wound layer. During the third stage, about the 8th day, the cellular extensions divide several times and elongate towards the surface. A new cortical or wound tissue is formed during the fourth stage beginning on the 12th day. The wound tissue is continuous with the old cortex within 3 weeks.
- Notes on the induced spawning and larval rearing of the Asian moon scallop, Amusium pleuronectes (Linné), in the laboratoryBelda, C. A.; Del Norte, A. G. C. (Elsevier, 1988-08)Sexual and chemical stimulations were effective in inducing the scallop, Amusium pleuronectes (Linné), to spawn sperm only, whereas thermal stimulation was not at all successful. Chemical stimulation consisted of serotonin injections into the ripe gonads. Fertilizable eggs were obtained by stripping gametes from the ovaries. Cleavage of zygotes (72.4 μm mean diameter) occured 1 h after fertilization. Trochophores (58.9–111.2 μm in length) and straight-hinge veligers or D-veligers (80.0–130.2 μm) were observed in less than 24 h. Five days after fertilization, the umbone stage (101.6–169.1 μm) was reached. Crawling pediveligers (174.0–250.0 μm) appeared after 9 days. Seawater in the culture vessels was changed 24 h after fertilization and every other day thereafter. The larvae were fed on Isochrysis galbana (Tahitian strain) at an initial concentration of 30 cells/gml.