Microbiome diversity and host immune functions influence survivorship of sponge holobionts under future ocean conditions

Abstract

The sponge-associated microbial community contributes to the overall health and adaptive capacity of the sponge holobiont. This community is regulated by the environment and the immune system of the host. However, little is known about the effect of environmental stress on the regulation of host immune functions and how this may, in turn, affect sponge–microbe interactions. In this study, we compared the bacterial diversity and immune repertoire of the demosponge, <i>Neopetrosia compacta</i>, and the calcareous sponge, <i>Leucetta chagosensis</i>, under varying levels of acidification and warming stress based on climate scenarios predicted for 2100. <i>Neopetrosia compacta</i> harbors a diverse microbial community and possesses a rich repertoire of scavenger receptors while <i>L. chagosensis</i> has a less diverse microbiome and an expanded range of pattern recognition receptors and immune response-related genes. Upon exposure to RCP 8.5 conditions, the microbiome composition and host transcriptome of <i>N. compacta</i> remained stable, which correlated with high survival (75%). In contrast, tissue necrosis and low survival (25%) of <i>L. chagosensis</i> was accompanied by microbial community shifts and downregulation of host immune-related pathways. Meta-analysis of microbiome diversity and immunological repertoire across poriferan classes further highlights the importance of host–microbe interactions in predicting the fate of sponges under future ocean conditions.