National Committee on Marine Sciences (NCMS)
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- The genus Sargassum (Phaeophyta, Sargassaceae) from Balibago, Calatagan, PhilippinesAng, P. O.; Trono, G. C. (Walter de Gruyter GmbH, 1987)Eight species of Sargassum: S. baccularia (Mertens) C. Agardh, S. cinctum J. Agardh, S. crassifolium J. A-gardh, S. ilicifolium (Turner) C. Agardh, S. notarisii Zanardini, S. oligocystum Montagne, S. paniculatum J. Agardh, and S. siliquosum J. Agardh are reported from Balibago, Calatagan, Philippines. Growth and changes in the morphology of the two most common species, S. paniculatum and S. siliquosum, were noted throughout their different growth phases over 1 1/2 years. Collections of material representing growth stages of the other species were made at periodic intervals. The more consistent morphological characters for each species were recognized and their utility in taxonomic work was discussed. These characters include outline and length/width ratio of the leaves characteristic of each branching order of laterals, shape and size of the vesicles, muricatous nature of the terminal branchlets, branching pattern of the receptacles, morphology of the female receptacles and the type of holdfast.
- Laboratory and field growth studies of commercial strains of Eucheuma denticulatum and Kappaphycus alvarezii in the PhilippinesDawes, Clinton J.; Lluisma, A. O.; Trono, G. C. (Springer, 1994-02)Daily growth rates of 0.1 to 8.4% d-1 for the brown form and 0.2 to 6.3% d-1 of the green form were measured for 3 to 5-cm long branches of the tropical red seaweed Kappaphycus alvarezii cultured in the laboratory. Highest growth rates were found using inexpensive enrichments such as soil water and coconut water supplemented with 0.7 mM N and 13 µM P and with a liquid fertilizer, Algafer, produced from seaweeds in the Philippines. Laboratory grown branches of both K. alvarezii and Eucheuma denticulatum transplanted to rafts in the field showed daily growth rates of 4.4 to 8.9% d-1, as high or higher than other reported growth rates. The studies, carried out in the Philippines, demonstrate the viability and high yield of laboratory cultivars and methods to keep laboratory culture costs low.
- Clonal propagation of Eucheuma denticulatum and Kappaphycus alvarezii for Philippine seaweed farmsDawes, C. J.; Trono, G. C.; Lluisma, A. O. (Springer, 1993-06)Technique improvement and cost reduction of branch culture, micropropagation, and callus production of carrageenan-yielding seaweeds Kappaphycus alvarezii and Eucheuma denticulatum is presented. Low cost branch culture is possible by enriching seawater with 0.1% coconut water with 1 mg l−1 indole-3-butyric acid for 24 h wk−1 or continuous culture with 0.01% Algafer, a Philippine fertilizer. Micropropagation of 0.5 cm explants had almost 100% new branch production demonstrating the viability of callus regenerated plants. The use of carrageenan as a media for callus production was not effective when compared to agar. Propagules of both species, transferred from the University of the Philippine Marine Science Institute (UPMSI) culture facility to the field, showed daily percent growth rates of 5 to 5.5% d−1 over 84 days. Based on the costs of the UPMSI laboratory, a culture facility in the seaweed farming area is estimated to cost about U. S. $22000 during the initial year and 58% less the second year.