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National Committee on Marine Sciences (NCMS)

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1989 - 198911990 - 19931

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AGROVOC Keyword: search.filters.agrovoc.seaweedsAuthor: search.filters.author.Dawes, C. J.

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    Wound healing in cultured Eucheuma alvareziivar. tambalang Doty
    Azanza-Corrales, R.; Dawes, C. J. (Walter de Gruyter GmbH, 1989)
    Wound healing in segments of Eucheuma alvarezii var. tambalang grown in enriched media in the laboratory can be divided into four stages based on histological and cytological changes. During the first stage, approximately 2—4 days after wounding, proteinaceous and phenolic substances concentrate on pit plugs of cells adjacent to the wounded surface. In the second stage, about the sixth day, cellular extensions are produced from the pits of medullary and cortical cells of sub-wound layer. During the third stage, about the 8th day, the cellular extensions divide several times and elongate towards the surface. A new cortical or wound tissue is formed during the fourth stage beginning on the 12th day. The wound tissue is continuous with the old cortex within 3 weeks.
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    Clonal propagation of Eucheuma denticulatum and Kappaphycus alvarezii for Philippine seaweed farms
    Dawes, C. J.; Trono, G. C.; Lluisma, A. O. (Springer, 1993-06)
    Technique improvement and cost reduction of branch culture, micropropagation, and callus production of carrageenan-yielding seaweeds Kappaphycus alvarezii and Eucheuma denticulatum is presented. Low cost branch culture is possible by enriching seawater with 0.1% coconut water with 1 mg l−1 indole-3-butyric acid for 24 h wk−1 or continuous culture with 0.01% Algafer, a Philippine fertilizer. Micropropagation of 0.5 cm explants had almost 100% new branch production demonstrating the viability of callus regenerated plants. The use of carrageenan as a media for callus production was not effective when compared to agar. Propagules of both species, transferred from the University of the Philippine Marine Science Institute (UPMSI) culture facility to the field, showed daily percent growth rates of 5 to 5.5% d−1 over 84 days. Based on the costs of the UPMSI laboratory, a culture facility in the seaweed farming area is estimated to cost about U. S. $22000 during the initial year and 58% less the second year.