Subong, Bryan John J.Lluisma, Arturo O.Azanza, Rhodora V.Salvador-Reyes, Lilibeth A.2025-06-222020-12-23Subong, B. J. J., Lluisma, A. O., Azanza, R. V., & Salvador-Reyes, L. A. (2020). Differentiating two closely related <i>Alexandrium</i> species using comparative quantitative proteomics. <i>Toxins, 13</i>(1), 7. https://doi.org/10.3390/toxins130100072072-665110.3390/toxins13010007https://hdl.handle.net/20.500.14697/580<i>Alexandrium minutum</i> and <i>Alexandrium tamutum</i> are two closely related harmful algal bloom (HAB)-causing species with different toxicity. Using isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics and two-dimensional differential gel electrophoresis (2D-DIGE), a comprehensive characterization of the proteomes of <i>A. minutum<i> and <i>A. tamutum<i> was performed to identify the cellular and molecular underpinnings for the dissimilarity between these two species. A total of 1436 proteins and 420 protein spots were identified using iTRAQ-based proteomics and 2D-DIGE, respectively. Both methods revealed little difference (10–12%) between the proteomes of <i>A. minutum</i> and <i>A. tamutum</i>, highlighting that these organisms follow similar cellular and biological processes at the exponential stage. Toxin biosynthetic enzymes were present in both organisms. However, the gonyautoxin-producing <i>A. minutum</i> showed higher levels of osmotic growth proteins, Zn-dependent alcohol dehydrogenase and type-I polyketide synthase compared to the non-toxic A. tamutum</i>. Further, <i>A. tamutum</i> had increased S-adenosylmethionine transferase that may potentially have a negative feedback mechanism to toxin biosynthesis. The complementary proteomics approach provided insights into the biochemistry of these two closely related HAB-causing organisms. The identified proteins are potential biomarkers for organismal toxicity and could be explored for environmental monitoring.enProteomicsAlexandriumMarine toxinsArticleSDG 14 - Life below waterproteomicsred tidesPyrrophycophytaChallenge 1: Understand and beat marine pollutionChallenge 2: Protect and restore ecosystems and biodiversity