Concepcion, GiselaFoderaro, Tommaso A.Eldredge, Glenn S.Lobkovsky, EmilClardy, JonBarrows, Louis R.Ireland, Chris M.2025-05-041995-10Concepción, G. P., Foderaro, T. A., Eldredge, G. S., Lobkovsky, E., Clardy, J., Barrows, L. R., & Ireland, C. M. (1995). Topoisomerase II-mediated DNA cleavage by adocia- and xestoquinones from the Philippine sponge <i>Xestospongia</i> sp. <i>Journal of Medicinal Chemistry, 38</i>(22), 4503–4507. https://doi.org/10.1021/jm00022a0160022-26231520-480410.1021/jm00022a016https://hdl.handle.net/20.500.14697/399Investigation of an orange <i>Xestospongia</i> sp. sponge collected at Cape Bolinao in northern Luzon, Philippines, yielded the known compounds adociaquinones A and B (1, 2) and six new metabolites, secoadociaquinones A and B (3, 4), 14-methoxyxestoquinone (5), 15-methoxyxestoquinone (6), 15-chloro-14-hydroxyxestoquinone (7), and 14-chloro-15-hydroxyxestoquinone (8). All compounds showed inhibition of topoisomerase II in catalytic DNA unwinding and/or decatenation assays. Furthermore, adociaquinone B showed activity in a KSDS assay, suggesting it inhibits the enzyme by freezing the enzyme-DNA cleavable complex. Interestingly, adociaquinone B did not displace ethidium bromide from DNA or unwind supercoiled DNA, implying it does not intercalate DNA.enSpongesDNA topoisomerase IIArticleSDG 14 - Life below waterSpongiaDNA cleavageChallenge 9: Skills, knowledge, and technology for all